Autoradiography: techniques and application by Renato Baserga

Cover of: Autoradiography: techniques and application | Renato Baserga

Published by Hoeber Medical Division, Harper & Row in New York .

Written in English

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Subjects:

  • Autoradiography.

Edition Notes

Includes bibliographies.

Book details

Statement[by] Renato Baserga [and] Daniel Malamud.
SeriesModern methods in experimental pathology
ContributionsMalamud, Daniel, 1921- joint author.
Classifications
LC ClassificationsQH324.9.A9 B37 1969
The Physical Object
Paginationxii, 281 p.
Number of Pages281
ID Numbers
Open LibraryOL5695103M
LC Control Number70085826

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A detailed, practical guide to light and electron microscopic applications in quantitative studies; investigation of DNA, RNA, and protein synthesis; and other medical and botanical areas. Reagents in an appendix. Author and subject indexes.

For. TY - BOOK. T1 - Autoradiography. T2 - Techniques and applications. AU - Baserga, R. AU - Malamud, Daniel. PY - Y1 - M3 - Book. BT - AutoradiographyCited by: Autoradiography: techniques and application. New York, Hoeber Medical Division, Harper & Row [©] (OCoLC) Online version: Baserga, Renato.

Autoradiography: techniques and application. New York, Hoeber Medical Division, Harper & Row [©] (OCoLC) Document Type: Book: All Authors / Contributors: Renato Baserga; Daniel.

This is a PDF-only article. The first page of the PDF of this article appears : R. Carter. Purchase Autoradiography and Immunocytochemistry - 1st Edition. Print Book & E-Book. ISBNFormat: Ebook. Autoradiography is a detection method in which X-ray or photographic film is exposed to emissions from radioisotopes on TLC plates to produce an image on the film.

After exposure (exposure time depends on the amount of radioactivity per zone), the film is developed to reveal the location of the areas of radioactivity as darkened spots or zones of varying optical density. Identification of Single Neurons by Intracellular Application of Tracers.

Peter Schubert. Pages Autoradiography: techniques and application book Freeze-Fracturing and Autoradiography Techniques: Freeze-Fracture Autoradiography.

Schiller, R. Taugner This book is an impressive witness to. Imaging: Autoradiography and Phosphorimaging (Protocol summary only for purposes of this preview site) Many of the commonly used techniques in molecular cloning depend on methods to map accurately the distribution of radioactive atoms on two-dimensional (2D) surfaces.

The book's scope includes 2D and 3D X-ray imaging techniques from soft-X-ray to megavoltage energies, including computed tomography, fluoroscopy, dental imaging and small animal imaging, with several chapters dedicated to breast imaging techniques.

2D and 3D industrial imaging is incorporated, including imaging of artworks. This book is organized into 10 parts.

The introductory part provides basic information on cells and tissues and outlines the methodology in cellular pathology techniques. This is followed by chapters that deal with various aspects of cellular pathology including tissues, cells and cell products of special interests, electron microscopy, and.

Physical Techniques in Biological Research, Second Edition, Volume III, Part B: Autoradiography at the Cellular Level focuses on high resolution autoradiography with tritium. This Autoradiography: techniques and application book reviews the different autoradiographic techniques and difficulties in theoretical principles needed for the interpretation of autoradiograms.

The Cell: Biochemistry, Physiology, Morphology, Volume I focuses on the advancements of processes, techniques, and approaches used in studies on the structure and functions of cells. The selection first offers information on the optical methods in cytology, fixation and staining, and autoradiography.

Discussions focus on microscopical analysis, techniques related to autoradiography. Analysing the hybrid fragments using autoradiography.

DNA Fingerprinting Applications. As discussed earlier the technique of fingerprinting is used for DNA analysis in forensic tests and paternity tests.

Apart from these two fields, it is also used in determining the frequency of a particular gene in a population which gives rise to diversity. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Keywords. The book further benefits from an increased number of photographs that illustrate typical results and techniques - all in full colour.

Cellular Pathology 3rd edition reflects the current requirements of cellular pathology teaching and practice and provides essential reading on any course that relates to cellular pathology, histology and. Autoradiography is the study of biological events in tissue sections using radioactivity.

Autoradiography permits the localization of radioactive substances in tissues by means of emitted radiation effects on photographic emulsions. DNA Fingerprinting (DNA Profiling) Procedure and Applications As fingerprints have proved useful for identification of individuals to fairly good extent, DNA of an individual distinguishes her or him more exactly from others and using DNA for distinguishing and identification is DNA fingerprinting.

COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus.

The "old fashioned" techniques of microscopy and paraffin sectioning have become indispensable. The fact that the instruction manuals are dated has resulted in this manual. This Laboratory Manual in Plant Microtechnique started from a series of handouts from my course at Berkeley in Plant Microtechnique.

Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Show all. A probe is a DNA or RNA fragment of variable length which can be labelled by a radioactive molecule. It hybridises to its complementary DNA and can be detected by autoradiography. Q How can the gene expression be controlled with the help of RNA. A The gene. The autoradiography cassettes are excellent for film contact and prevention of light leakage.

It features a new lightweight alloy with a lead shiel. view product Autoradiography Cassette is an extremely cost-effective solution for every lab performing autoradiography and phosphor imaging applications, these. Autoradiography or photographing or fluorescence detection Different samples of equal quantity are loaded in each lane.

From the blot it is clear that protein expression (40, MW) is more in lane 4 and 5. Applications: Highly sensitive method to detect a specific protein even in very low quantity.

Used in clinical diagnosis. Gel electrophoresis is a basic technique used to separate DNA, RNA or proteins. It is a common starting point for many biotechnology experiments and is often paired with the blotting techniques (see below).

This technique relies on electricity to separate out molecules in an agarose gel, a. Discover the best Electron Microscope books and audiobooks. Learn from Electron Microscope experts like Elsevier Books Reference and Elsevier Books Reference.

Read Electron Microscope books like Materials Science and Engineering of Carbon and Colloidal Gold with a free trial. For instance, autoradiography on whole sections of experimental anima yields information on the site and rate of accumulation, while typical techniques used in metabolic studies can be used to follow the rate and products of metabolism.

(B) Analytical Applications: 1. Enzyme and Ligand Binding Studies. These readily reproducible techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET).

Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal s: 1.

Techniques for Extracellular Recordings.- "Blind" patch-clamp recordings from rodent hippocampal slices.- Whole-cell patch clamp recordings.- Section 8: Immunohistochemistry and autoradiography.- Analysis of Receptor Binding And Quantitative Autoradiography.- Techniques in Immunohistochemistry and immunocytochemistry   The book's scope includes 2D and 3D X-ray imaging techniques from soft-X-ray to megavoltage energies, including computed tomography, fluoroscopy, dental imaging and small animal imaging, with several chapters dedicated to breast imaging techniques.

2D and 3D industrial imaging is incorporated, including imaging of artworks. Hyphenated techniques: Principles and applications of GC-MS, LC-MS and LC-NMR techniques for the study of natural products.

Computer assisted structure elucidation of natural products 6. Spectroscopic elucidation of structures of morphine (alkaloid), abietic acid (diterpenoid), glycyrritinic acid (triterpenoid), diosgenin (steroidal sapogenin), rutin (flavonoid), and xanthotoxin (coumarin).

Although several techniques exist to diminish motion artifacts, a few accounts for both tissue displacement and changes in density due to the compression and dilation of the lungs, which cause quantification errors. 18F autoradiography With the Mimosa Characterization and Application expanding field that utilizes nonequilibrium.

Applications. In biology, this technique may be used to determine the tissue (or cell) localization of a radioactive substance, either introduced into a metabolic pathway, bound to a receptor or enzyme, or hybridized to a nucleic acid.

Applications for autoradiography are broad, ranging from biomedical to environmental sciences to industry. Nagata T () Techniques and applications of electron microscopic radio- autography in biomedical research. In Mukherjee TMM, Swift JG, eds. World Health Organization Training Course on Electron Microscopy in Biomedi- cal Research and Diagnosis of Human Diseases.

Adelaide, Australia, Univer- sity of Adelaide Press,   Autoradiography, which can also be digital, is used in many cases for biological and medical applications. In contrast to other methods of detecting radiation, they can show the locations of radioactive materials in a sample.

The images can therefore be used with biological specimens labeled with such materials, to track cellular activity for. Abstract. This book consists of three parts, each containing several papers. The part headings are: Techniques of Immunization and Hybrid Construction, Production and Modification of Antibodies and Lymphokines, and Applications of Monoclonal Lymphoid Products.

27 Specialized Techniques. Lymphocyte Preparation. Autoradiography. Time-Lapse Recording. Cell Synchrony. Culture of Cells from Poikilotherms. Somatic Cell Fusion. Production of Monoclonal Antibodies.

28 Training Programs. Objectives. Preparative and Manipulative Skills. Basic Cell Culture. The technique of monolayer freeze-fracture autoradiography (MONOFARG) has been developed and the principles, quantitation, and application of the method are described.

Cell monolayers attached to polylysine- treated glass were freeze-fractured, shadowed, and coated with dry, Parlodion-supported Ilford L4 photographic emulsion at room temperature. Discover the best Staining books and audiobooks.

Learn from Staining experts like GuruKPO and Elsevier Books Reference. Read Staining books like Microbiology Lab Manual and Quantitative and Qualitative Microscopy with a free trial. The filter is pre treated to prevent non-specific binding of the probe to the filter.

The filter is then treated with appropriate radioactive single stranded DNA probe under condition favouring hybridization. Filter is then washed repeatedly washed to remove free probe. The hybridized probes are detected by autoradiography.

Nuclear techniques can now provide factual evidence to help classify collections of objects as well as accurate information on dates of production or ultimate geographical source. Of particular value is the fact that such new techniques provide chemical analyses of samples that are much smaller than those required previously.Medical books Cellular Pathology.

It describes the complete range of histological techniques utilised in the diagnosis of disease and in medical research. The author introduces and explains the techniques available, the advantages of each one compared to others, and their application in cellular pathology.In autoradiography, labeled material is allowed Most research applications of radioisotopes, at some stage, require Techniques have been developed for apply-ing these corrections, and a great deal of research has been carried out to improve the efficiency of counting, using various detection systems.

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